Proteins involved in endocytic transit (Vacuolin pg1)

Vacuolin pg1 text

Top: Vacuolin (purple dots), a protein related to flotillin/reggie from vertebrates is a marker for late, neutral endosomes.

Right: Fluid-phase-marker (red) reaches the GFP-vacuolin decorated endosome after one hour.

Vacuolin pg1 Text 2

Function: Cells lacking the gene encoding vacuolin B have a single giant neutral endosome and a prolonged transit of endocytic cargo. Vacuolin A mutants are phenotypically normal. Vacuolin has also been used to target cytoskeletal proteins to the late endosome to analyse the function of the actin coat (click here for more).


Rauchenberger et al., 1997

  • Rauchenberger, R., Hacker, U., Murphy, J., Niewöhner, J. & Maniak, M.: Coronin and vacuolin identify consecutive stages of a late, actin-coated endocytic compartment in Dictyostelium. Current Biology 7, 215-218, 1997

Jenne et al., 1998

  • Jenne, N., Rauchenberger, R., Hacker, U., Kast, T. & Maniak, M.: Targeted gene disruption reveals a role for vacuolin B in the late endocytic pathway and exocytosis. J. Cell Sci. 111, 61-70, 1998.