Development of shape specific polymeric nanoparticles and study of their dispersion and toxicology after pulmonary application in murine lung (NanoImprint Pharmakon, NIP)
In the past the encapsulation of therapeutical agents in polymeric particles was successfully used for developing new carrier systems for drug targeting (determining the location of action) as well as controlled drug release (determining the release duration). Up to the present the most interesting particle parameters for manipulation and optimization of the in vivo behavior (in living organism) of such an agent carrier particle are the design parameters like particle size, polymer type and surface chemistry.
The design parameter particle shape, which also is assumed to have a great influence on the in vivobehavior, has not been analyzed attentively up to now. This is due to the limited techniques available for producing non-spherical particles. The unique physicochemical properties of such particles however, provide distinct advantages as novel carrier systems. Thus first studies show that a rational design of the carrier systems allows control over the in vivo behavior, so the cellular uptake can be optimized by selective particle shape variation of the carrier system. Until now only few attempts to produce shape specific polymeric nanoparticle carrier systems have been described.
The key technology which provides a monodisperse distribution of polymeric nanoparticles and a high reproducibility accompanied by a sufficient quantity for following cell experiments is the NanoImprint Technology. This field of research, especially 3D imprint, is established at the INA and also carried out with very high resolution in other research projectshere. With NanoImprint Technology the design parameters such as size and material as well as shape can be adjusted precisely and varied in a wide spectrum. Thus the task of the INA in the NIP project will be the development and optimization of a process for producing polymeric nanoparticles of different shapes and sizes as well as the characterization of the nanoparticles with respect to their physical properties
The biopharmaceutical analysis (in vivo/vitro) and medical studies with external lungs of animals (ex vivo) will be performed in collaboration with groups of the University Medical Center Giessen-Marburg and Justus-Liebig-University of Giessen.
This project is based on a collaboration between the Institute of Pharmaceutical Technologies and Biopharmacy (Faculty of Pharmacy 16 , Philipps-University Marburg, Germany), the department of Internal Medicine (Justus-Liebig-University of Giessen, University Hospital Giessen and Marburg) and the Institute of Nanostructure Technologies and Analytics (INA, University of Kassel, Germany).
Additional topics regarding to Functional 3D Particles: Magnetic nanoparticles (Prof. Ehresmann, Group of thin layers and synchrotron radiation, University of Kassel); Development of novel Self-Aligned NanoShaping (SANS) Technique for Imprinting full 3D shaping of Micro- and Nanoparticles.
S. Reuter ; A. Istock ; M. Smolarczyk ; U.-M. Ha ; O. Schneider ; L. Gomer ; H. Hillmer: Fabrication of 2D and 3D Shaped Micro- and Nanoparticles via Substrate Conformal Imprint Lithography. In: Technical Digest of 13th Int. Conf. Nanoimprint and Nanoprint Technology (NNT2014) (2014), 34P-4-22
S. Schudy ; L. Gomer ; M.A. Smolarczyk ; T. Sharbatitehrani ; H. Hillmer: Replica Molding Techniques for Large Area Application of Nanoimprint with Soft Stamps. In: ICMAT 2013 (2013), Symposium M, ICMAT 13-A-1536
S. Schudy ; M.A. Smolarczyk ; L. Gomer ; A.T. Istock ; O. Schneider ; N. Worapattrakul ; F. Pilger; H. Hillmer: Polymer nanoparticles shaped in two and three dimensions by substrate conformal imprint lithography. In: ICMAT 2013 (2013), Symposium H, ICMAT 13-A-1528
S. Schudy ; M. A. Smolarcyk ; H. H. Hillmer ; N. Worapattrakul ; F. Pilger: Nano-Formgebungsstruktur. Patent: DE 102011054789 A1 (2011), WO 2013/060618 A1 (2011), EP 2771161 (2014).
Additional information about Patent: http://www.gino-innovativ.de/de/patente.asp - Eintrag: TE_015 3D-Nanoabformung