MICROSEED

The MICROSEED research project is investigating the effects of the seed microbiome on the development of plant microbiomes in roots, rhizosphere and leaves - particularly in the context of soils with different microbial characteristics from organic and conventional agriculture. The focus is on the early colonization of the plant and the vertical transfer of microbes from seed to plant.

Background

While soil and rhizosphere microbiomes have been intensively studied, the seed microbiome has received little attention so far. Yet it plays a crucial role in early microbial colonization and plant health. The project considers the role of seeds in the context of the entire holobiont and aims to contribute to the development of resilient cropping systems.

Experimental approach

In a controlled greenhouse experiment, lettuce(Lactuca sativa) is grown as a model plant in soil from adjacent organically and conventionally farmed plots. Two factors are combined:

• Seed treatment: untreated (natural microbiome) vs. surface sterilized
• Soil type: organic vs. conventionally farmed

Microbiome samples from seeds, soil, rhizosphere, roots and leaves are analyzed by amplicon sequencing (16S/ITS) and network analyses.

Aims

• To elucidate the role of the seed microbiome in initial plant colonization

• Evaluation of the influence of soil management on these processes

• Identification of microorganisms that are stably transferred from seed to plant

Funding

The project is funded by the Central Research Fund (ZFF) of the University of Kassel.