Aufsatz in einer Fachzeitschrift


Carmona-Gutierrez, D., Bauer, M.A., Zimmermann, A., Aguilera, A., Austriaco, N., et al (2018) ‘Guidelines and recommendations on yeast cell death nomenclature’, Microbial Cell, 5(1), 4–31.


Klassen, R., Schaffrath, R. (2017) ‘Role of Pseudouridine Formation by Deg1 for Functionality of Two Glutamine Isoacceptor tRNAs’, BIOMOLECULES, 7(1), 8.
Sokołowski, M., Klassen, R., Bruch, A., Schaffrath, R., Glatt, S. (2017) ‘Cooperativity between different tRNA modifications and their modification pathways’, BBA - Biochimica et Biophysica Acta, TBD.
Mehlgarten, C., Prochaska, H., Hammermeister, A., Abdel-Fattah, W., Wagner, M., Krutyholowa, R., Jun, S.E., Kim, G.-T., Glatt, S., Breunig, K.D., Stark, M.J.R., Schaffrath, R. (2017) ‘Use of a Yeast tRNase Killer Toxin to Diagnose Kti12 Motifs Required for tRNA Modification by Elongator’, Toxins, 9(9), 272.
Schaffrath, R., Leidel, S.A. (2017) ‘Wobble uridine modifications-a reason to live, a reason to die?!’, RNA Biology, 14(9), 1209–1222.
Schaffrath, R., Klassen, R. (2017) ‘Combined tRNA modification defects impair protein homeostasis and synthesis of the yeast prion protein Rnq1’, Prion, 11(1), 48–53.
Klassen, R., Bruch, A., Schaffrath, R. (2017) ‘Independent suppression of ribosomal+1 frameshifts by different tRNA anticodon loop modifications’, Rna Biology, 14(9), 1252–1259.


Klassen, R., Ciftci, A., Funk, J., Bruch, A., Butter, F., Schaffrath, R. (2016) ‘tRNA anticodon loop modifications ensure protein homeostasis and cell morphogenesis in yeast’, Nucleic Acids Research, 44(22), 10946–10959.
Jüdes, A., Bruch, A., Klassen, R., Helm, M., Schaffrath, R. (2016) ‘Sulfur transfer and activation by ubiquitin-like modifier system Uba4 center dot Urm1 link protein urmylation and tRNA thiolation in yeast’, Microbial Cell, 3(11), 423–433.
Inigo, S., Durand, A.N., Ritter, A., Le Gall, S., Termathe, M., Klassen, R., Tohge, T., De Coninck, B., Van Leene, J., De Clercq, R., Cammue, B.P.A., Fernie, A.R., Gevaert, K., De Jaeger, G., Leidel, S.A., Schaffrath, R., Van Lijsebettens, M., Pauwels, L., Goossens, A. (2016) ‘Glutaredoxin GRXS17 Associates with the Cytosolic Iron-Sulfur Cluster Assembly Pathway’, Plant Physiology, 172(2), 858–873.
Schaffrath, R., Klassen, R. (2016) ‘Killerplasmid-codierte Ribotoxin-RNasen aus Hefen’, BIOspektrum, 22(1), 16–18.


Abdel-Fattah, W., Jablonowski, D., Di Santo, R., Scheidt, V., Hammermeister, A., ten Have, S., Thüring, K.L., Helm, M., Schaffrath, R., Stark, M. (2015) ‘Phosphorylation of Elp1 by Hrr25 is required for Elongator-dependent tRNA modification in yeast’, Plos Genetics, 2015, e1004931, available:
Jüdes, A., Ebert, F., Bär, C., Thüring, K.L., Harrer, A., Klassen, R., Helm, M., Stark, M., Schaffrath, R. (2015) ‘Urmylation and tRNA thiolation functions of ubiquitin-like Uba4.Urm1 systems are conserved from yeast to man’, FEBS Letters, 589(8), 904–909.
Kast, A., Voges, R., Schröder, M., Schaffrath, R., Klassen, R., Meinhardt, F. (2015) ‘Auto-selection of cytoplasmic yeast virus like elements encoding toxin/anti-toxin systems involves a nuclear barrier for immunity gene expression’, Plos Genetics, 11, e1005005.
Klassen, R., Grunewald, P., Thüring, K.L., Eichler, C., Helm, M., Schaffrath, R. (2015) ‘Loss of anticodon wobble uridine modifications affects tRNALys function and protein levels in Saccharomyces cerevisiae’, Plos One, 11/2015, e0119261, available:


Schaffrath, R., Stark, M. (2014) ‘Decoding the biosynthesis and function of diphthamide, an enigmatic modification of translation elongation factor 2 (EF2)’, Microbial Cell, 1, 203–205.
Schaffrath, R., Abdel-Fattah, W., Klassen, R., Stark, M. (2014) ‘The diphthamide modification pathway from Saccharomyces cerevisiae – Revisited’, Molecular Microbiology, 94, 1213–1226, available:
Jun, S.E., Cho, K.-H., Hwang, J.-Y., Abdel-Fattah, W., Hammermeister, A., Schaffrath, R., Bowman, J.L., Kim, G.-T. (2014) ‘Comparative Analysis of the Conserved Functions of Arabidopsis DRL1 and Yeast KTI12’, 2014, 1–8, available:
Scheidt, V., Jüdes, A., Bär, C., Klassen, R., Schaffrath, R. (2014) ‘Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling’, Microbial Cell, 1, 416–424, available:


Uthman, S., Bär, C., Liu, S., ten Have, S., Giorgini, F., Stark, M., Schaffrath, R., Scheidt, V. (2013) ‘The Amidation Step of Diphthamide Biosynthesis in Yeast Requires DPH6, a Gene Identified through Mining the DPH1-DPH5 Interaction Network’, Plos Genetics, 9, e1003334, available:
Abdel-Fattah, W., Scheidt, V., Uthman, S., Stark, M., Schaffrath, R. (2013) ‘Insights into Diphthamide, Key Diphtheria Toxin Effector’, Toxins, 5, 958–968, available:
Woodacre, A., Jablonowski, D., Lone, M., Schneiter, R., Giorgini, F., Schaffrath, R. (2013) ‘A novel Sit4 phosphatase complex is involved in the response to ceramide stress in yeast’, Oxidative Medicine and Cellular Longevity, 2013, 129645, available:


Meineke, B., Schwer, B., Schaffrath, R., Shuman, S. (2011) ‘Determinants of eukaryal cell killing by the bacterial ribotoxin PrrC’, Nucleic Acids Research, 39, 687–700, available:


Mehlgarten, C., Jablonowski, D., Wrackmeyer, U., Tschitschmann, S., Sondermann, D., Jäger, G., Gong, Z., Byström, A., Schaffrath, R., Breunig, K.D. (2010) ‘Elongator function in tRNA wobble uridine modification is conserved between yeast and plants’, Molecular Microbiology, 76, 1082–1094, available:;jsessionid=DEFA9ED3496A34F118A613CAB9AF7475.f02t01.


Jablonowski, D., Täubert, J.-E., Stark, M., Bär, C., Schaffrath, R. (2009) ‘Distinct Subsets of Sit4 Holophosphatases Are Required for Inhibition of Saccharomyces cerevisiae Growth by Rapamycin and Zymocin’, Eukaryotic Cell, 8, 1637–1647, available:
Mehlgarten, C., Jablonowski, D., Breunig, K.D., Stark, M., Schaffrath, R. (2009) ‘Elongator function depends on antagonistic regulation by casein kinase Hrr25 and protein phosphatase Sit4’, Molecular Microbiology, 73, 869–881, available:


Zabel, R., Bär, C., Mehlgarten, C., Schaffrath, R. (2008) ‘Yeast alpha-tubulin suppressor Ats1/Kti13 relates to the Elongator complex and interacts with Elongator partner protein Kti11’, Molecular Microbiology, 69, 175–187.
Studte, P., Zink, S., Jablonowski, D., Bär, C., von der Haar, T., Tuite, M.F., Schaffrath, R. (2008) ‘tRNA and protein methylase complexes mediate zymocin toxicity in yeast’, Molecular Microbiology, 69, 1266–1277, available:
Bär, C., Zabel, R., Liu, S., Stark, M., Schaffrath, R. (2008) ‘A versatile partner of eukaryotic protein complexes that is involved in multiple biological processes: Kti11/Dph3’, Molecular Microbiology, 69, 1221–1233, available:
Nandakumar, J., Schwer, B., Schaffrath, R., Shuman, S. (2008) ‘RNA repair: An antidote to cytotoxic eukaryal RNA damage’, Molecular Cell, 31, 278–286, available:


Jablonowski, D., Schaffrath, R. (2007) ‘Zymocin, a composite chitinase and tRNase killer toxin from yeast’, Biochemical Society Transactions, 35, 1533–1537.
Mehlgarten, C., Zink, S., Rutter, J., Schaffrath, R. (2007) ‘Dosage suppression of the Kluyveromyces lactis zymocin by Saccharomyces cerevisiae ISR1 and UGP1’, Fems Yeast Research, 7, 722–730, available:


Chen, Z., Zhang, H., Jablonowski, D., Zhou, X., Ren, X., Hong, X., Schaffrath, R., Gong, Z., Zhu, J.-K. (2006) ‘Mutations in ABO1/ELO2, a subunit of holo-elongator, increase abscisic acid sensitivity and drought tolerance in Arabidopsis thaliana’, Molecular and Cellular Biology, 26, 6902–6912, available:
Klassen, R., Jablonowski, D., Stark, M., Schaffrath, R., Meinhardt, F. (2006) ‘Mating-type locus control of killer toxins from Kluyveromyces lactis and Pichia acaciae’, Fems Yeast Research, 6, 404–413, available:
Jablonowski, D., Zink, S., Mehlgarten, C., Daum, G., Schaffrath, R. (2006) ‘tRNA(Glu) wobble uridine methylation by Trm9 identifies Elongator’s key role for zymocin-induced cell death in yeast’, Molecular Microbiology, 59, 677–688, available:


Zink, S., Mehlgarten, C., Kitamoto, H.K., Nagase, J., Jablonowski, D., Dickson, R.C., Stark, M., Schaffrath, R. (2005) ‘Mannosyl-diinositolphospho-ceramide, the major yeast plasma membrane sphingolipid, governs toxicity of Kluyveromyces lactis zymocin’, Eukaryotic Cell, 4, 879–889, available:


Jablonowski, D., Stark, M., Fichtner, L., Schaffrath, R. (2004) ‘The yeast elongator histone acetylase requires Sit4-dependent dephosphorylation for toxin-target capacity’, Molecular Biology of the Cell, 15, 1459–1469, available:
Mehlgarten, C., Schaffrath, R. (2004) ‘After chitin docking, toxicity of Kluyveromyces lactis zymocin requires Saccharomyces cerevisiae plasma membrane H+-ATPase’, Cellular Microbiology, 6, 569–580, available:


Schaffrath, R. (2003) ‘Kluyveromyces lactis Zymocin: Model für mikrobielle Kompetition und Proliferation’, Biospektrum, 9, 168–169.
Fichtner, L., Jablonowski, D., Schierhorn, A., Kitamoto, H.K., Stark, M., Schaffrath, R. (2003) ‘Elongator’s toxin-target (TOT) function is nuclear localization sequence dependent and suppressed by post-translational modification’, Molecular Microbiology, 49, 1297–1307, available:
Frohloff, F., Jablonowski, D., Fichtner, L., Schaffrath, R. (2003) ‘Subunit communications crucial for the functional integrity of the yeast RNA polymerase II elongator (gamma-toxin target (TOT)) complex’, Journal of Biological Chemistry, 278, 956–961, available:
Mehlgarten, C., Schaffrath, R. (2003) ‘Mutant casein kinase I (Hrr25p/Kti14p) abrogates the G1 cell cycle arrest induced by Kluyveromyces lactis zymocin in budding yeast’, Molecular Genetics and Genomics, 269, 188–196.


Fichtner, L., Frohloff, F., Bürkner, K., Breunig, K.D., Larsen, M., Schaffrath, R. (2002) ‘Molecular analysis of KTI12/TOT4, a Saccharomyces cerevisiae gene required for Kluyveromyces lactis zymocin action’, Molecular Microbiology, 43, 783–791, available:
Kitamoto, H.K., Jablonowski, D., Nagase, J., Schaffrath, R. (2002) ‘Defects in yeast RNA polymerase II transcription elicit hypersensitivity to G1 arrest induced by Kluyveromyces lactis zymocin’, Molecular Genetics and Genomics, 268, 49–55.
Jablonowski, D., Schaffrath, R. (2002) ‘Saccharomyces cerevisiae RNA polymerase II is affected by Kluyveromyces lactis zymocin’, Journal of Biological Chemistry, 277, 26276–26280, available:
Fichtner, L., Frohloff, F., Jablonowski, D., Stark, M., Schaffrath, R. (2002) ‘Protein interactions within Saccharomyces cerevisiae Elongator, a complex essential for Kluyveromyces lactis zymocicity’, Molecular Microbiology, 45, 817–826, available:
Fichtner, L., Schaffrath, R. (2002) ‘KTI11 and KTI13, Saccharomyces cerevisiae genes controlling sensitivity to G1 arrest induced by Kluyveromyces lactis zymocin’, Molecular Microbiology, 44, 865–875, available:
Klassen, R., Jablonowski, D., Schaffrath, R., Meinhardt, F. (2002) ‘Genome organization of the linear Pichia etchellsii plasmid pPE1A: evidence for expression of an extracellular chitin-binding protein homologous to the alpha-subunit of the Kluyveromyces lactis killer toxin’, Plasmid, 47, 224–233, available:


Jablonowski, D., Fichtner, L., Martin, V.J., Klassen, R., Meinhardt, F., Stark, M., Schaffrath, R., Martin, V. (2001) ‘Saccharomyces cerevisiae cell wall chitin, the Kluyveromyces lactis zymocin receptor’, Yeast, 18, 1285–1299, available:
Schaffrath, R., Meacock, P.A. (2001) ‘An SSB encoded by and operating on linear killer plasmids from Kluyveromyces lactis’, Yeast, 18, 1239–1247.
Frohloff, F., Fichtner, L., Jablonowski, D., Breunig, K.D., Schaffrath, R. (2001) ‘Saccharomyces cerevisiae Elongator mutations confer resistance to the Kluyveromyces lactis zymocin’, Embo Journal, 20, 1993–2003, available:
Jablonowski, D., Butler, A.R., Fichtner, L., Gardiner, D., Schaffrath, R., Stark, M. (2001) ‘Sit4p protein phosphatase is required for sensitivity of Saccharomyces cerevisiae to Kluyveromyces lactis zymocin’, Genetics, 159, 1479–1489, available:
Jablonowski, D., Frohloff, F., Fichtner, L., Stark, M., Schaffrath, R. (2001) ‘Kluyveromyces lactis zymocin mode of action is linked to RNA polymerase II function via Elongator’, Molecular Microbiology, 42, 1095–1105, available:


Schaffrath, R., Meacock, P.A., Sasnauskas, K. (2000) ‘Use of gene shuffles to study the cytoplasmic transcription system operating on Kluyveromyces lactis linear DNA plasmids’, Enzyme and Microbial Technology, 26, 664–670, available:
Schaffrath, R., Breunig, K.D. (2000) ‘Genetics and molecular physiology of the yeast Kluyveromyces lactis’, Fungal Genetics and Biology, 30, 173–190, available:


Schaffrath, R., Meinhardt, F., Meacock, P.A. (1999) ‘Genetic manipulation of Kluyveromyces lactis linear DNA plasmids: gene targeting and plasmid shuffles’, Fems Microbiology Letters, 178, 201–210.


Schaffrath, R., Meinhardt, F. (1998) ‘Das Killersystem von Kluyveromyces lactis: Molekularbiologie und Biotechnik’, Biospektrum, 4, 40–42.


Meinhardt, F., Schaffrath, R., Larsen, M. (1997) ‘Microbial linear plasmids’, Applied Microbiology and Biotechnology, 47, 329–336.
Schaffrath, R., Meinhardt, F., Meacock, P.A. (1997) ‘ORF7 of yeast plasmid pGHL2: Analysis of gene expression in vivo’, Current Genetics, 31, 190–192.
Schaffrath, R., Stark, M., Struhl, K. (1997) ‘Zymocin-mediated G(1) cell cycle arrest: Analysis of killer toxin insensitive (kti) Saccharomyces erevisiae mutants’, European Journal of Cell Biology, 72, 111.
Schaffrath, R., Stark, M., Struhl, K. (1997) ‘Toxin-mediated cell cycle arrest in yeast: the killer phenomenon of Kluyveromyces lactis’, BIOforum International, 1, 83–85.


Schaffrath, R., Meacock, P.A. (1996) ‘A cytoplasmic gene-shuffle system in Kluyveromyces lactis: Use of epitope tagging to detect a killer plasmid-encoded gene product’, Molecular Microbiology, 19, 545–554, available:
Schaffrath, R., Meinhardt, F., Meacock, P.A. (1996) ‘Yeast killer plasmid pGKL2: Molecular analysis of UCS5, a cytoplasmic promoter element essential for ORF5 gene function’, Molecular & General Genetics, 250, 286–294.
Sharan, R.J., Schaffrath, R., Laltanpuia, L., Schneeweiß, F. (1996) ‘Radiotoxicity of chronic ingestion of tritiated water on liver HMG proteins of swiss albino mice’, Proceedings of the National Academy of Sciences, India, Section B (Biological Sciences), 1996, 5–10.


Schaffrath, R., Soond, S.M., Meacock, P.A. (1995) ‘The DNA and RNA polymerase genes of yeast plasmid pGKL2 are essential loci for plasmid integrity and maintenance’, Microbiology, 141, 2591–2599, available:
Schaffrath, R., Soond, S.M., Meacock, P.A. (1995) ‘Cytoplasmic gene expression in yeast - A plasmid-encoded transcription system in Kluyveromyces lactis’, Biochemical Society Transactions, 23, S128–S128.
Schaffrath, R., Meacock, P.A. (1995) ‘Kluyveromyces lactis killer plasmid pGKL2: Molecular analysis of an essential gene, ORF5’, Yeast, 11, 615–628.


Schaffrath, R., Stark, M., Meinhardt, F., Gunge, N. (1992) ‘Kluyveromyces lactis killer system: ORF1 of pGKL2 has no function in immunity expression and is dispesable for killer plasmid replication and maintenance’, Current Genetics, 21, 357–363.

Beitrag in einem Sammelband


Schaffrath, R., Meinhardt, F., Klassen, R. (2018) ‘Yeast Killer Toxins: Fundamentals and ApplicationsYeast Killer Toxins: Fundamentals and Applications’, in Anke, T. and Schüffler, A., eds., Physiology and Genetics: Selected Basic and Applied Aspects, Springer International Publishing: Cham, 87–118.
Klassen, R., Schaffrath, R., Buzzini, P., Ganter, P.F. (2018) ‘Antagonistic Interactions and Killer Yeasts’, in Buzzini, P., Lachance, M.-A. and Yurkov, A., eds., Yeasts in Natural Ecosystems: Ecology, Springer International Publishing: Cham, 229–275.


Eichler, C., Herberg, F.W., Schmidt, F., Schaffrath, R. (2013) ‘Does the bee locus encode an additional or alternative pilus in Enterococcus faecalis?’, in Mendez-Vilas, A., ed., Worldwide Research Efforts in the Fighting Against Microbial Pathogens: From Basic Research to Technological Developments, BrownWalker Press: Boca Raton, FL, USA, 134–138.


Uthman, S., Liu, S., Giorgini, F., Stark, M., Costanzo, M., Schaffrath, R. (2012) ‘Diphtheria disease and genes involved in formation of diphthamide, key effector of the diphtheria toxin’, in Kumar, R., ed., Insight and Control of Infectious Disease in Global Scenario, INTECH Open Access Publisher: Rijeka, Croatia, 333–356, available:


Kheir, E., Bär, C., Jablonowski, D., Schaffrath, R. (2011) ‘Cell growth control by tRNase ribotoxins from bacteria and yeast’, in Mendez-Vilas, A., ed., Science and Technology Against Microbial Pathogens. Research, Development and Evaluation, World Scientific Publishing Co. Pte. Ltd: Singapore, 398–402, available:
Uthman, S., Kheir, E., Bär, C., Jablonowski, D., Schaffrath, R. (2011) ‘Growth inhibition strategies based on antimicrobial microbes/toxins.’, in Mendez-Vilas, A., ed., Science Against Microbial Pathogens: Communicating Current Research and Technological Advances, Formatex Research Center: Badajoz, Spain, 1321–1329, available:


Schaffrath, R., Meinhardt, F. (2005) ‘Kluyveromyces lactis zymocin and other plasmid-encoded yeast killer toxins’, in Schmitt MJ, S.R., ed., Microbial Protein Toxins, Springer-Verlag: Berlin, Heidelberg, New York, 133–155.


Fichtner, L., Jablonowski, D., Frohloff, F., Schaffrath, R. (2003) ‘Phenotypic analysis of the Kluyveromyces lactis killer phenomenon’, in Wolf, K.B., Barth G, eds., Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology, Springer-Verlag: Berlin, Heidelberg, 179–183.
Schaffrath, R. (2003) ‘Genetic manipulation of the Kluyveromyces lactis killer plasmids k1 and k2’, in Wolf, K.B., Barth G, eds., Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology, Springer-Verlag: Berlin, Heidelberg, 185–190.
Jablonowski, D., Fichtner, L., Frohloff, F., Schaffrath, R. (2003) ‘Chitin binding capability of the zymocin complex from Kluyveromyces lactis’, in Wolf, K.B., Barth G, eds., Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology, Springer-Verlag: Berlin, Heidelberg, 191–194.


Meinhardt, F., Schaffrath, R. (2001) ‘Extranuclear inheritance: cytoplasmic linear double-stranded DNA killer elements of the dairy yeast Kluyveromyces lactis’, in Esser, K., Lüttge, U., Kadereit, J. and Beyschlag, W., eds., Progress in Botany, Springer-Verlag: Berlin, Heidelberg, 51–70.



Schmitt, M.J., Schaffrath, R. (Eds.) (2005) Microbial Protein Toxins, Springer-Verlag: Berlin, Heidelberg, New York.

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