DFG - SFB 394


Koordiniert durch:Prof. Dr. rer. nat. L. M. G. Heilmeyer, Bochum, Deutschland
Förderungszeitraum:von Jan 1996 bis Jan 2002
Projektwebseite: gepris.dfg.de

Structures and Mechanisms in Biological Signal and Energy Transduction

The goal of the collaborative research centre 394 is to determine three-dimensional structures of proteins in order to describe function and dynamic movements on an atomic level. A special aspect is the description of the domain interactions of different proteins. Thus force development by structural muscle proteins and their regulation or iontransport by membrane proteins or interactions of phosphorylated amino acids with proteins shall be explained.
An array of methods will be employed like proteincristallography, fouriertransforminfraredspectroscopy, electronresonancespectroscopy, transient kinetics, realtime measurements of interactions by surface plasmon resonance and microcalorimetry. In addition laserforceps will be developed combined with a single fluorophor-polarisation detection which will allow to determine interactions of domains of different proteins on a single-molecule level.
The collaborative research centre is composed of 21 projects of which 16 are located at the Ruhr-Universität Bochum including two central service projects for peptide analysis by masspectrometry and DNA analysis and 5 are located at the Max-Planck-Institut for Molecular Physiology at Dortmund.